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The Department of Genetic Medicine is seeking a Research Specialist II who will independently work in translational epigenetics research lab to identify, develop, and optimize assays and protocols. Designs and independently implements experimental approaches for research projects. Perform a wide range of sophisticated assays, including preparation of samples for next-generation sequencing approaches (i.e., RNA-seq and chromatin immunoprecipitation followed by sequencing/ChIP-seq).
Requires significant and important contributions to various aspects of research efforts, including troubleshooting, independent decision-making, and training/guidance of other lab personnel including staff and students. Assists with writing of manuscripts and creating figures for publication and grants.
Specific Duties & Responsibilities:
Scientific Responsibilities (90%)
- Experimental Responsibilities
- Basic laboratory methods/techniques associated with genetics and molecular biology such as: isolation of DNA and RNA, PCR, quantitative real-time PCR, DNA electrophoresis, DNA cloning, DNA sequencing, quantitative and qualitative analyses of DNA, RNA, and protein molecules, as well as other functional and enzymatic assays.
- Performs basic and advanced cell culture techniques, including maintaining induced pluripotent stem cell-derived models.
- Uses light/fluorescence/confocal microscope and computer programs to analyze data.
- Prepares buffers and solutions/reagents using knowledge of chemistry formulation.
- Contributes to the maintenance of the laboratory, equipment, and supplies; conducts routine safety tests.
- Independently performs various higher-level assays, including Chip-qPCR and library preparation for the next-generation sequencing techniques of RNA-seq, ChIP-seq, and whole genome-bisulfite sequencing.
- Maintains careful records of all samples obtained and experiments performed.
- Routinely and independently assesses the quality of generated data.
- Prepares data for and performs statistical analyses.
- Presents summaries and quality metrics of data generated at regular intervals in laboratory meetings.
- Animal Model Responsibilities
- Independently maintains mouse colonies; genotyping/weighing of mice.
- Intra-peritoneal injections and other minor procedures on mice.
- Setting up timed mating for embryonic developmental stage-specific investigations.
- Dissection of mice for anatomical and histological studies.
- Harvesting and preparing tissues, particularly bone and brain, for molecular and phenotypic analyses and independently performing these analyses.
- Uses scientific knowledge and lab experience to make judgments and decisions. Works independently.
- Makes scientific contribution to protocol development, efficiency, and productivity.
- Collaboratively and independently develops new techniques.
- Applies published research methods to current experimental work by investigating published protocols and discussing them with PI.
- Scientifically contributes to writing of manuscripts and grants.
- Process analyzed data into figures for scientific publications and presentations
Administrative Responsibilities (10%)
- Responsible for budget management. Manages budgetary duties by ensuring charges, invoices, and orders are correctly routed to the proper grant.
- Responsible for daily ordering of all laboratory consumables, reagents, supplies, and equipment; maintains financial records.
- Responsible for equipment inventories and maintenance, and maintaining service contracts on all equipment.
- Ensures all laboratory protocols and biosafety approvals are up-to-date.
- Responsible for overall organization, upkeep, and operational elements of laboratory, functioning as a representative for the PI.
- Acts as a project manager for the lab. Coordinates with all other supporting staff units including administration and IT.
- Exercises independent, managerial judgment and responsibility for decisions and results.
- Helps to hire, supervise, and train incoming laboratory staff.
- Advises personnel on proper laboratory techniques and use of laboratory equipment.
- Advises personnel on the proper maintenance of laboratory notebooks and electronic files.
- Ensures the laboratory and staff comply with annual safety inspections and safety office recommendations.
Minimum Qualifications (Mandatory):
- Requires BS/BA, or equivalent, in Biology, Chemistry or Physics.
- Must have a working knowledge of one or more of the following disciplines achieved through undergraduate studies and laboratory experience: biology, biochemistry, chemistry, physics, microbiology and/or genetics.
- The candidate must be quantitatively inclined.
- 2 years laboratory experience performing relevant techniques.
- Master’s degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.*
*JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.
* Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.
Special Knowledge, Skills & Abilities:
- Experience with molecular biology (including advanced) techniques, cell culture, and mouse work (as above).
- Experience with chromatin immunoprecipitation, library preparation for RNA-seq and next-generation sequencing, and some knowledge of bioinformatics is preferred.
Graduate student; subject to change.