EPA Office/Lab and Location: Two research opportunities are available at the Environmental Protection Agency (EPA), Office of Research and Development (ORD), Center for Computational Toxicology and Exposure (CCTE), Great Lakes Toxicology Ecology Division (GLTED) located in either Research Triangle Park, North Carolina or Cincinnati, Ohio.
Research Project: The Agency is faced with significant challenges related to characterizing the modes of action of chemicals, the effects of exposures to mixtures of chemicals, and in finding ways to group chemicals that facilitates read-across from data rich to data poor chemicals. The Molecular Indicators Branch within the Office of Research and Development is addressing these challenges through the characterization of molecular responses in aquatic organisms exposed to chemicals. In large part, MIB targets transcriptomic and epigenetic responses using next generation sequencing (NGS)-based tools (RNA-seq, reduced representation bisulfite sequencing – RRBS) to develop biomarkers that can discriminate chemical classes and provide an understanding of impacted biological processes and pathways. There are also opportunities to explore and analyze other omics data including metabolomic and proteomic data from whole animal responses and multigenerational studies. The research participant will be involved in designing of laboratory experiments, NGS data analysis and data interpretation, design and development of new statistical methods for integrated omics data analysis, and in the communication of results intra- and extramurally. The research participant will be trained in molecular genetics, bioinformatics, statistics, machine learning algorithms for big data analytics, and advanced statistical analysis of complex omics data sets.
With guidance from the mentor, the research participant may be involved in the following training activities:
- Developing statistical methods and tools to analyze NGS-based gene expression and epigenetic data
- Designing analysis pipelines and performing analysis of DNA-seq,RNA-seq, ChIP-seq, RRBS, other omics environmental exposure data
- Providing biological interpretation of exposure data to characterize chemical MOA
- Applying machine learning algorithms for identification of biomarkers and classification of environmental toxicants
- Contributing to the preparation of peer-reviewed journal articles and disseminating research results to project partners, stakeholders, and the research community
- Presenting research at regional, national, and/or international conferences and workshops
Learning Objectives: The research participant will be afforded the opportunity to interact with internationally recognized leaders, both within and outside the EPA, the area of bioinformatics and in ecotoxicogenomics. The research participant will have the opportunity to contribute to and to publish original research. This training opportunity will provide an early career scientist with knowledge, skills, and abilities needed to apply new technologies and associated data to regulatory decision-making at the local, national, or international scale and to pursue a professional career in life sciences research.
Appointment Length: The appointment will initially be for one year and may be renewed three to four additional years upon EPA recommendation and subject to availability of funding.
Level of Participation: The appointment is full-time.
EPA Security Clearance: Completion of a successful background investigation by the Office of Personnel Management (OPM) is required for an applicant to be on-boarded at EPA.
ORISE Information: This program, administered by ORAU through its contract with the U.S. Department of Energy (DOE) to manage the Oak Ridge Institute for Science and Education (ORISE), was established through an interagency agreement between DOE and EPA. Participants do not become employees of EPA, DOE or the program administrator, and there are no employment-related benefits. Proof of health insurance is required for participation in this program. Health insurance can be obtained through ORISE.